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Created and analyzed library of 10,000 gene mutations in Arabidopsis, resulting in a worldwide resource for plant genomic research.
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Created and analyzed library of 10,000 gene mutations in Arabidopsis, resulting in a worldwide resource for plant genomic research.
EXPLORE >   Projects >  Functional Genomics of Arabidopsis
Functional Genomics of Arabidopsis
OBJECTIVES
TEAM
APPROACH
IMPACT
INTELLECTUAL PROPERTY
Objectives
The aim of this project was to study gene function in the model plant Arabidopsis thaliana using a combination of transgenic cell lines, gene expression profiling and bioinformatics.
Project Information
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Started: 2002
Ended: 2006

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Team
This project involves eight research groups in the Department of Botany at the University of Toronto. Together, they formed the Arabidopsis Research Group (ARG), which was established to provide resources and expertise for the Arabidopsis community in Canada. The research team collaborated with Performance Plants, an Ontario-based plant biotechnology company.
Collaborator Role In Project Organization Country
John Coleman
Collaborator
University of Toronto (UofT)
Canada
Peter McCourt
Collaborator
University of Toronto (UofT)
Canada


Approach
The group employed green-fluorescent protein (GFP) transgenes, hormone-regulated promoters, Affymetrix GeneChips and bioinformatics tools; and, to study spatial, temporal and cell-specific behaviors of gene expression, an enhancer-trap system with GFP as a reporter gene was used on approximately 5000 lines of Arabidopsis.
Project Information
Website
Started: 2002
Ended: 2006

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Impact
By the end of the project, the group had generated two new resources to examine gene function: the first included 5000 GFP-enhancer trap lines, which were used to study temporal-spatial gene expression patterns; the second included 10 000 transgenic strains carrying hormone-regulated promoters, which were used to study how abnormal gene expression affects cell function and plant development. Transcription profiling was also performed to generate approximately 300 gene expression experiments using the Botany Array Resource—the first plant transcript profiling facility built in Canada.

The group has also established the Botany Bioinformatics Cluster, a supercomputer developed for this project and used for storing and analyzing the information it produced. For example, to date, the group has generated approximately 1000 datasets and 24.6 million expression experiments. This data has been shared internationally and the group is recognized as a major contributor to The Arabidopsis Information Resource (TAIR). This project has also provided new opportunities for commercial interaction and technological development. In summary, the information, reagents and equipment developed by this project have generated and will continue to generate important information that will assist in the understanding various plants including agriculturally-related species.
Project Information
Website
Started: 2002
Ended: 2006

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Intellectual Property

Project Information
Website
Started: 2002
Ended: 2006

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CATEGORIES
Application Area
Agriculture
Core Technology
Nucleic acids: Gene expression systems, Microarrays
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