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Overview
My interests primarily lie in the development and deployment of novel genomics technologies to advance human health research. Discovery and validation applications for DNA, RNA and protein are all areas in which I am involved. A few key projects I am involved with are:
RNA amplification
Working with Dr. Norman Iscove of the Ontario Cancer Institute, I have been involved in the development of a novel protocol for the global amplification of RNA from minute cell populations (1-10 cells). This technique has been adapted to work with a novel Agilent microarray we have created, as well as to standard array platforms. It has proven useful in several of our other areas of focus detailed below.
In vitro fertilisation
Our ability to amplify RNA from small samples has allowed us the fortune of working with Drs. Ellen Greenblatt (Centre for Fertility and Reproductive Science; CFRS), Ted Brown (SLRI), Crystal Chan (CFRS, UofT), Allan King (U Guelph) and Pavneesh Madan (U Guelph). We are working to identify markers that can be used to predict embryo competence and endometrial receptivity in both humans and cows.
Prostate Cancer
In collaboration with the Prostate Cancer research group at the University Health Network (Drs. Neil Fleshner, Joan Sweet, Susan Done) and Dr. Ted Brown (SLRI), we have been working to understand the complex relationship of tumor stroma and epithelium. Again our ability to profile minute cell quantities has enabled this project allowing us to work from LCM samples from very small biopsies.
Protein Arrays
We are also working on ways to make protein arrays that are easy to manufacture, store and utilise. We are working on this both through our own efforts as well as in collaboration with Dr. Sirano Dhe-Paganon (Structural Genomics Consortium).
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Researcher Information
Head of Operations
Microarray Centre
Website
101 College Street
9-312, TMDT
Toronto, Ontario
Canada M5G 1L7
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Current Position
- Head of Operations, UHN Microarray Centre
Previous Positions: - Consultant, Pacific Growth Equities (2006-2007)
- Consultant, Infinium Securities Inc (2006)
- Manager of Commercialisation and External Contracts, UHN Microarray Centre (1999-2002)
- Research Technician, Ontario Cancer Institute (1998-1999)
- Research Associate, Allelix Biopharmaceuticals (1998)
Boards and Committees: - Scientific Advisory Board, Functional Genomics Data Society (2010-Present)
- Scientific Advisory Board, Microarray and Gene Expression Data Society (2006-2010)
- Functional Genomics Symposium Organiser (2010)
- Ovarian Cancer Conference Trainees Session Co-Organiser (2010)
- Scientific Advisory Board, Drugs2Discovery, IBC Conferences (2007-2009)
- Board of Directors, Microarray and Gene Expression Data Society (2005-2006)
- Meeting Organiser, 7th International MGED meeting (2007)
Patents: - Canadian Patent CA2316045, Devices and methods for producing microarrays of biological samples
- Canadian Patent Applicaiton CA2322086, Methods for production of microarrays of biological samples
- US Patent 6,902,702, Devices and methods for producing microarrays of biological samples
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Researcher Information
Head of Operations
Microarray Centre
Website
101 College Street
9-312, TMDT
Toronto, Ontario
Canada M5G 1L7
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Schumacher, A; Kapranov, P; Kaminsky, Z; Flanagan, J; Assadzadeh, A; Yau, P; Virtanen, C; Winegarden, N; Cheng, J; Gingeras, T; Petronis, A. 2006. Microarray-based DNA methylation profiling: technology and applications. NUCLEIC ACIDS RESEARCH 34 (2): 528-542.
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Heisler, LE; Torti, D; Boutros, PC; Watson, J; Chan, C; Winegarden, N; Takahashi, M; Yau, P; Huang, THM; Farnham, PJ; Jurisica, I; Woodgett, JR; Bremner, R; Penn, LZ; Der, SD. 2005. CpG Island microarray probe sequences derived from a physical library are representative of CpG Islands annotated on the human genome. NUCLEIC ACIDS RESEARCH 33 (9): 2952-2961.
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Iscove, NN; Barbara, M; Gu, M; Gibson, M; Modi, C; Winegarden, N. 2002. Representation is faithfully preserved in global cDNA amplified exponentially from sub-picogram quantities of mRNA. NATURE BIOTECHNOLOGY 20 (9): 940-943..
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Chang, WY; Winegarden, NA; Paraiso, JP; Stevens, ML; Westwood, JT. 2000. Visualization of nascent transcripts on Drosophila polytene chromosomes using BrUTP incorporation. BIOTECHNIQUES 29 (5): 934-936.
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Researcher Information
Head of Operations
Microarray Centre
Website
101 College Street
9-312, TMDT
Toronto, Ontario
Canada M5G 1L7
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ORGANIZATIONS
PROJECTS
RESOURCES
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OTHER INFORMATION
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Researcher Information
Head of Operations
Microarray Centre
Website
101 College Street
9-312, TMDT
Toronto, Ontario
Canada M5G 1L7
YOU MAY ALSO BE INTERESTED IN:
CATEGORIES
Application Area
Animal health,
Human health,
Instrumentation and tools
Disciplinary Focus
Clinical science,
Experimental biology and chemistry
Research Paradigm
Focused-scope projects,
Large-scale projects,
Technology development
Core Technology
Nucleic acids:
Gene expression systems,
Genotyping,
Microarrays,
RNA technologies
Proteins:
Protein chips,
Protein expression and purification,
Protein-protein interaction assays
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