The SIDNET shRNA archive consists of human and mouse pGIPZ lentiviral shRNAmir-GFP libraries developed by Open Biosystems in collaboration with Dr. Greg Hannon the Cold Spring Harbour Laboratories and Dr. Steve Elledge of Harvard University. Each library contains more than 62,000 constructs containing microRNA-adapted shRNA combined with the pGIPZ †vector capable of triggering the production of RNAi in a variety of cell types. The inclusion of TurboGFP and shRNAmir allow for the visual marking of shRNAmir expressing cells. Additionally, pGIPZ shRNA can be packaged into lentiviral particles for transduction into difficult cell lines, including primary and non-dividing cells. pGIPZ shRNAmir gene families allow the convenience of Gene ontology (GO) annotated collections targeting all genes in a particular gene family or biological pathway. These collections will greatly simplify pathway analysis, target identification and validation.